- Cell Line: NCI-H292
- Synonyms:
- NCI-H292
- NCIH292
- H292
- H-292
- NCI-HUT-292
- Hut292
- Culture Type: Cell line
- Keywords: Human pulmonary mucoepidermoid carcinoma cell line
- Description: Established from lymph node metastasis of a pulmonary mucoepidermoid carcinoma of the lung from a 32 year-old black female
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 32 years
- Ethnicity: Black
- Gender: Female
- Smoking habit:-
- Tissue (origin): Lung
- Disease: Mucoepidermoid Carcinoma of the Lung (LUMEC)
- Primary/Metastasis: Metastatic (Collection Site: lymph node)
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent, grown as monolayer
- Life span: Infinite (continuous culture)
- Karyotype: near-diploid, modal chromosome number 47
Doubling time: Approximately 48 hrs - Microsatellite instability: Instable
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: yes, produce tumors in nude mice
- Gene expression:
- mucicarmine positive
- keratin positive
- vimentin positive
- negative for neurofilament triplet protein
- L-DOPA decaroxylase negative
- Sequence variations:
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-001075?tab=mutations
- https://depmap.org/portal/cell_line/ACH-001075?tab=fusions
- For information, please visit
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time: Semi confluent culture (~ 80%)
- Seeding density:-
- Recommended split ratio: 1:3 to 1:8
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO??
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% DMSO??
- Applications:
- Cancer research
- 3D cell culture
- High-throughput screening
- Toxicology
- Virus replication studies (Hepatitis B virus)
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H292 cell image link (Source: DepMap)
- NCI-H292 cell image link 1 (Source: Cytion)
- NCI-H292 cell image link 2 (Source: Cytion)
- NCI-H292 cell image link 3 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.