Cell Line: NCI-H292

  • Cell Line: NCI-H292
  • Synonyms:
    • NCI-H292
    • NCIH292
    • H292
    • H-292
    • NCI-HUT-292
    • Hut292
  • Culture Type: Cell line
  • Keywords: Human pulmonary mucoepidermoid carcinoma cell line
  • Description: Established from lymph node metastasis of a pulmonary mucoepidermoid carcinoma of the lung from a 32 year-old black female
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 32 years
      • Ethnicity: Black
      • Gender: Female
      • Smoking habit:-
    • Tissue (origin): Lung
    • Disease: Mucoepidermoid Carcinoma of the Lung (LUMEC)
    • Primary/Metastasis: Metastatic (Collection Site: lymph node)
    • Year of origin:-
  • Cell line features:
    • Cell Type: Epithelial
    • Morphology: Epithelial-like
    • Growth mode: Adherent, grown as monolayer
    • Life span: Infinite (continuous culture)
    • Karyotype: near-diploid, modal chromosome number  47
      Doubling time: Approximately 48 hrs
    • Microsatellite instability: Instable
    • Special features:
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity: yes, produce tumors in nude mice
    • Gene expression:
      • mucicarmine positive
      • keratin positive
      • vimentin positive
      • negative for neurofilament triplet protein
      • L-DOPA decaroxylase negative
    • Sequence variations:
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-001075?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-001075?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: RPMI-1640 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: Humidified 37°C incubator
      • Atmosphere: 5% CO2, 95% Air
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
      • Harvesting time: Semi confluent culture (~ 80%)
      • Seeding density:-
      • Recommended split ratio: 1:3 to 1:8
      • Medium Renewal: Every 2 to 3 days
    • Cryopreservation:
      • Freezing medium: Complete growth medium +  5% DMSO??
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • Cancer research
    • 3D cell culture
    • High-throughput screening
    • Toxicology
    • Virus replication studies (Hepatitis B virus)
  • Availability:

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