- Cell Line: NULLI-SCC1
- Synonyms:
- Nulli SCC1
- Culture Type: Cell line
- Keywords: Mouse Teratocarcinoma Cell Line
- Description: Established from Teratocarcinoma tumor of mice, strain129/Sv
- Origin:
- Organism:
- Species: Mouse (Mus musculus)
- Age:-
- Strain: 129/Sv
- Gender: Male
- Species: Mouse (Mus musculus)
- Tissue (origin): Testis
- Disease: Teratocarcinoma tumor
- Primary/Metastasis:-
- Collection site:-
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Undifferentiated
- Morphology:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:
- Doubling time:-
- Microsatellite instability:-
- Special features: Can grow on gelatin-coated dishes
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:-
- Cell Type: Undifferentiated
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM (with 4.5 g/l glucose) + 10% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:5
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% (v/v) DMSO
- Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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