Cell Line: N3.36

  • Cell Line: N3.36
  • Synonyms: N3-36
  • Culture Type: Cell line
  • Keywords:  Mouse Hybridoma (anti-fiber/myosin)
  • Description: Established by fusion of P3X63Ag8.653 mouse myeloma cells with spleen cells from a mouse that had been immunized with partially purified human neonatal (5 days postpartum) skeletal muscle myosin heavy chain.
  • Origin:
    • Organism:
      • Species: Mouse (Mus musculus)
      • Age:-
      • Ethnicity:-
      • Gender:-
    • Tissue (origin): Spleen cell and mouse myeloma cell hybrid
    • Year of origin:-
  • Cell line features:
    • Cell Type: Hybridoma (Spleen cell and mouse myeloma cell hybrid)
    • Morphology: Lymphoblast-like
    • Growth mode: single round cells, lightly adherent
    • Life span: Infinite (continuous culture)
    • Karyotype: Murine hypertetraploid karyotype, 20% polyploidy
    • Doubling time: ~ 24 hours
    • Special features:-
    • Product: Secretion of a IgM monoclonal antibody (against neonatal and adult fast myosin heavy chain (MyHC))
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:
    • Sequence variations:-
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: DMEM (4.5 g/L glucose) + 0.1 mM nonessential amino acids + 1 mM sodium pyruvate + 10% FBS
    • Culture conditions: 
      • Temperature: Humidified 37°C incubator
      • Atmosphere: 5% CO2, 95% Air
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: 1 x 106 cells/ml
      • Seeding density: 0.2 x 106 cells/ml
      • Recommended split ratio: 1:5 to 1:10
      • Medium Renewal: Every 2 to 3 days
    • Cryopreservation:
      • Freezing medium:
        • Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • Antibody production
  • References
  • Images:
  • Abbreviations
    • BSL (Biosafety level)
    • FBS (Fetal Bovine Serum)
    • DMSO (Dimethyl sulfoxide)
    • DMEM (Dulbecco’s modified Eagle’s medium)
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