- Cell Line: N3.36
- Synonyms: N3-36
- Culture Type: Cell line
- Keywords: Mouse Hybridoma (anti-fiber/myosin)
- Description: Established by fusion of P3X63Ag8.653 mouse myeloma cells with spleen cells from a mouse that had been immunized with partially purified human neonatal (5 days postpartum) skeletal muscle myosin heavy chain.
- Origin:
- Organism:
- Species: Mouse (Mus musculus)
- Age:-
- Ethnicity:-
- Gender:-
- Tissue (origin): Spleen cell and mouse myeloma cell hybrid
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Hybridoma (Spleen cell and mouse myeloma cell hybrid)
- Morphology: Lymphoblast-like
- Growth mode: single round cells, lightly adherent
- Life span: Infinite (continuous culture)
- Karyotype: Murine hypertetraploid karyotype, 20% polyploidy
- Doubling time: ~ 24 hours
- Special features:-
- Product: Secretion of a IgM monoclonal antibody (against neonatal and adult fast myosin heavy chain (MyHC))
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:-
- Cell Type: Hybridoma (Spleen cell and mouse myeloma cell hybrid)
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM (4.5 g/L glucose) + 0.1 mM nonessential amino acids + 1 mM sodium pyruvate + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 1 x 106 cells/ml
- Seeding density: 0.2 x 106 cells/ml
- Recommended split ratio: 1:5 to 1:10
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Antibody production
- Antibody production
- Availability:
- American Type Culture Collection (ATCC): Product link
- Developmental Studies Hybridoma Bank Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- DMEM (Dulbecco’s modified Eagle’s medium)
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