Category: Lab Notes: Biotechniques
Antigen Retrieval
Antigen retrieval is a crucial laboratory technique used in immunohistochemistry (IHC) and related staining methods to unmask antigenic sites (epitopes)…
Electron Microscopy
Electron microscopy (EM) is a powerful imaging technique that uses a beam of electrons instead of visible light to visualize…
Correlative NanoSIMS
Correlative NanoSIMS is an advanced analytical approach that integrates nanoscale secondary ion mass spectrometry (NanoSIMS) with complementary imaging and analytical…
Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS)
Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) is an advanced surface analysis technique that provides high-resolution chemical and isotopic imaging at…
Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)
Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) is a highly sensitive surface analysis technique that provides detailed chemical and molecular information…
ATP Assay
An ATP assay is a biochemical method used to measure the amount of adenosine triphosphate (ATP) in biological samples. ATP…
Fluorophore
A fluorophore is a fluorescent chemical compound that can absorb light at a specific wavelength and subsequently emit light at…
4Pi microscope
The 4Pi microscope is an advanced fluorescence microscopy technique designed to dramatically improve axial (z-axis) resolution beyond the diffraction limit…
Abbe Diffraction Limit
The Abbe diffraction limit is a fundamental concept in optical microscopy that describes the smallest resolvable feature size achievable with…
Diffraction-Limited System
A diffraction-limited system refers to an optical system whose resolution is restricted solely by the fundamental effects of diffraction, rather…
STED Microscopy
Stimulated Emission Depletion (STED) microscopy is a super-resolution fluorescence imaging technique that overcomes the diffraction limit of conventional light microscopy. …
Thin-Layer Chromatography (TLC)
Thin-Layer Chromatography (TLC) is a widely used planar chromatographic technique for separating and identifying compounds within a mixture. It is…
High-Performance Thin-Layer Chromatography (HPTLC)
High-Performance Thin-Layer Chromatography (HPTLC) is an advanced form of conventional thin-layer chromatography (TLC), designed to enhance separation efficiency, reproducibility, and…
Gas Chromatography – Mass Spectrometry (GC-MS)
Gas Chromatography – Mass Spectrometry (GC-MS) is a powerful analytical technique that combines the separation capability of gas chromatography with…
Liquid Chromatography – Mass Spectrometry (LC-MS)
Liquid Chromatography – Mass Spectrometry (LC-MS) is a powerful analytical technique that combines the physical separation capabilities of liquid chromatography…