Category: Lab Notes: Biotechniques
Ultraviolet-Visible Spectroscopy (UV-Vis Spectroscopy)
UV-Vis spectroscopy, or ultraviolet-visible spectroscopy, is a fundamental analytical technique that measures the absorption of light by molecules in the…
Near-Infrared Spectroscopy
Near-infrared (NIR) spectroscopy is a non-destructive analytical technique that studies the interaction of matter with electromagnetic radiation in the near-infrared…
Fluorescence Spectroscopy
Fluorescence spectroscopy is a highly sensitive analytical technique that measures the emission of light from molecules following excitation by electromagnetic…
Spectroscopy
Spectroscopy is the scientific study of the interaction between matter and electromagnetic radiation, used to analyze the composition, structure, and…
Crystallography
Crystallography is a fundamental scientific technique used to determine the three-dimensional arrangement of atoms in crystalline materials. This powerful method…
Spectroscope
A spectroscope is an optical instrument designed to analyze the properties of light by dispersing it into its constituent wavelengths,…
Spectroscopic Probes
Spectroscopic probes are specialized molecules or molecular groups that provide detailed information about chemical and physical properties of systems through…
Biuret Assay
The Biuret Assay, also known as Biuret Method, is a classical biochemical technique used for the quantification of proteins based…
Spectrophotometer
A spectrophotometer is a fundamental analytical instrument used in laboratories to measure the interaction of light with samples. This sophisticated…
Beer-Lambert Law
The Beer-Lambert law, also known as Beer’s law or the Beer-Lambert-Bouguer law, is a fundamental principle in spectroscopy that describes…
Tissue Clearing
Tissue clearing is a revolutionary technique in biological imaging that renders opaque tissues transparent, allowing researchers to visualize deep structures…
CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel)
CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel) is a groundbreaking tissue clearing method that transforms intact biological tissues…
CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis)
CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) is a comprehensive tissue clearing method that uses simple, hydrophilic chemicals…
DISCO (Tissue Clearing Method)
DISCO (3DISCO, iDISCO, uDISCO) represents a family of organic solvent-based tissue clearing methods that have revolutionized the way researchers visualize…
SCALE (Sorbitol/Calf serum/Alpha-lipoic acid/Ellipsenone)
SCALE (Sorbitol/Calf serum/Alpha-lipoic acid/Ellipsenone) is a hydrophilic tissue clearing method that achieves transparency through hyperhydration while preserving fluorescent proteins and…