Category: Lab Notes: Biotechniques
SeeDB (See Deep Brain)
SeeDB (See Deep Brain) is an optical clearing method that utilizes simple sugar solutions to render biological tissues transparent while…
Serial Two-Photon Tomography
Serial Two-Photon Tomography (STPT) is an advanced imaging technique that combines the high-resolution, depthpenetra-ting capabilities of two-photon microscopy with automated…
Optical Projection Tomography
Optical Projection Tomography (OPT) is a powerful imaging technique used to acquire detailed three-dimensional (3D) representations of biological samples, particularly…
Swept Confocally-Aligned Planar Excitation (SCAPE) Microscopy
Swept Confocally-Aligned Planar Excitation (SCAPE) microscopy is an advanced light-sheet imaging technique designed to rapidly capture high-resolution, volumetric data from…
Volume Imaging Electron Microscopy
Volume imaging electron microscopy refers to a collection of advanced electron microscopy (EM) techniques that enable the reconstruction of three-dimensional…
Oblique Plane Microscopy
Oblique Plane Microscopy (OPM) is an innovative fluorescence imaging technique that enables rapid, three-dimensional imaging of living specimens by illuminating…
Two-Photon Microscopy
Two-photon microscopy is an advanced fluorescence imaging technique that enables deep-tissue visualization with high spatial resolution and minimal photodamage. It…
Super-Resolution Microscopy
Super-resolution microscopy encompasses a group of advanced optical imaging techniques that surpass the resolution limits of conventional light microscopy, enabling…
Widefield Microscopy
Widefield microscopy is one of the most widely used and foundational techniques in optical microscopy. It involves illuminating the entire…
Laser Scanning Confocal Microscopy
Laser scanning confocal microscopy (LSCM) is a powerful fluorescence imaging technique that enables the acquisition of high-resolution, optically sectioned images…
Laser Scanning Vs Spinning Disk Confocal Microscopy
Criteria Laser Scanning Confocal Microscopy (LSCM) Spinning Disk Confocal Microscopy Remarks Principle Uses a single point laser beam scanned across…
Spinning Disk Confocal Microscopy
Spinning disk confocal microscopy is an advanced imaging technique that enables rapid and high-resolution three-dimensional imaging of fluorescently labeled specimens,…
Acoustic Microscopy
Acoustic microscopy is a non-destructive imaging technique that uses high-frequency sound waves—typically in the range of tens to hundreds of…
Multiphoton Microscopy
Multiphoton microscopy (MPM) is an advanced fluorescence imaging technique that enables high-resolution, three-dimensional visualization of biological specimens, particularly deep within…
Compound Microscope
A compound microscope is a fundamental optical instrument used to magnify small objects that are invisible to the naked eye….