Cell Line: A15

CELL LINE ORIGIN
OrganismRat (Rattus norvegicus) (Claisse et al., 1979)
Sex
Female (Claisse et al., 1979)
Strain
BD-IX (Claisse et al., 1979)
Age
?
Source/TissueBrain, Glioma
DiseaseBrain glioma induced transplacentally by ethylnitroso urea (Claisse et al., 1979)
Year of origin (publication date
1979 (Claisse et al., 1979)
CELL LINE FEATURES
Morphology?
Cell type?
Growth modeAdherent, Monolayer
Doubling time?
Life spanInfinite (continuous culture)
Transformed?
TumorigenicForm malignant astrocytic tumours after short latent periods syngeneric animals.
Drug resistance?
Products?
KaryotypeModal chromosome number: 42
GENE EXPRESSION
  
APPLICATIONS
  
CULTURE MAINTENANCE AND STORAGE
Culture conditions and handling
Biosafety Level2 REF (It may differ in your country, follow the guidelines of your institute/country)
Plating efficiency?
Temperature37°C
AtmosphereHumidified incubator, 95% air + 5% CO2
Base mediumDMEM
Complete culture mediumDMEM (+ 2mM Glutamine) + 15% FBS
Medium Renewal?
Subculturing procedure
Harvesting timeSubconfluent culture (70 – 80%)
Split methodTrypsin/EDTA
Split ratio 
Seeding density 
Split frequency 
Cryopreservation
Cryomedium

Culture medium + 20% FBS + 10% DMSO

Storage conditions
Liquid nitrogen vapor phase
AVAILABILITY / SOURCE

Public Health England Culture Collections

Product page link
 CELL LINE IDENTIFICATIONS
Synonyms
 
Remarks 
Related cell lines 

 

References

  • Claisse et al., 1979. Cellular heterogeneity in an ethylnitrosourea-induced glioma: malignancy, karyology and other properties of tumour cell types. Br J Exp Pathol. 1979 Apr; 60(2): 209–224. PMID-221002; Full Text Links:  PMC2041444
  • https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=93040110&collection=ecacc_gc

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