CELL LINE ORIGIN | |
Organism | Rat (Rattus norvegicus) (Claisse et al., 1979) |
Sex | Female (Claisse et al., 1979) |
Strain | BD-IX (Claisse et al., 1979) |
Age | ? |
Source/Tissue | Brain, Glioma |
Disease | Brain glioma induced transplacentally by ethylnitroso urea (Claisse et al., 1979) |
Year of origin (publication date | 1979 (Claisse et al., 1979) |
CELL LINE FEATURES | |
Morphology | ? |
Cell type | ? |
Growth mode | Adherent, Monolayer |
Doubling time | ? |
Life span | Infinite (continuous culture) |
Transformed | ? |
Tumorigenic | Form malignant astrocytic tumours after short latent periods syngeneric animals. |
Drug resistance | ? |
Products | ? |
Karyotype | Modal chromosome number: 42 |
GENE EXPRESSION | |
APPLICATIONS | |
CULTURE MAINTENANCE AND STORAGE | |
Culture conditions and handling | |
Biosafety Level | 2 REF (It may differ in your country, follow the guidelines of your institute/country) |
Plating efficiency | ? |
Temperature | 37°C |
Atmosphere | Humidified incubator, 95% air + 5% CO2 |
Base medium | DMEM |
Complete culture medium | DMEM (+ 2mM Glutamine) + 15% FBS |
Medium Renewal | ? |
Subculturing procedure | |
Harvesting time | Subconfluent culture (70 – 80%) |
Split method | Trypsin/EDTA |
Split ratio | |
Seeding density | |
Split frequency | |
Cryopreservation | |
Cryomedium | Culture medium + 20% FBS + 10% DMSO |
Storage conditions | Liquid nitrogen vapor phase |
AVAILABILITY / SOURCE | |
Public Health England Culture Collections | Product page link |
CELL LINE IDENTIFICATIONS | |
Synonyms | |
Remarks | |
Related cell lines |
References
- Claisse et al., 1979. Cellular heterogeneity in an ethylnitrosourea-induced glioma: malignancy, karyology and other properties of tumour cell types. Br J Exp Pathol. 1979 Apr; 60(2): 209–224. PMID-221002; Full Text Links: PMC2041444
- https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=93040110&collection=ecacc_gc
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.