Overview | |
| Organism | Fish – rainbow trout (Oncorhynchus tshawytscha) |
| Source/Tissue | Liver |
| Morphology | Fibroblastoid |
| Cell type | |
| Growth mode | Adherent, monolayer |
| Doubling time | ≈ 80 hours |
| Life span | Infinite, continuous culture |
| Karyotype | |
| Transformed | |
| Tumorigenic | |
| Drug resistance | |
| Biosafety Level | 1 REF |
| Gene Expression | |
| Products | |
| Applications | |
| Remarks | |
| Related cell lines |
Cell line isolation information
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Recommended propagation method
Culture medium: 90% MEM (with Earle’s salts) + 10% heat inactivated FBS
Culture conditions: CO2, 20°C.
Split ratio: 1:2
Split frequency: Every 7-10 days
Harvesting time: Confluent culture
Split Method: Trypsin/EDTA
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ?
Availability
- DSMZ : Catalog No. : Product link
REFERENCES
- Ahne, 1985. Use of fish cell cultures for toxicity determination in order to reduce and replace the fish tests. Zentralbl Bakteriol Mikrobiol Hyg B. 180(5-6), 480-504. PMID-4024776
- DSMZ
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