Overview | |
Organism | Fish – rainbow trout (Oncorhynchus tshawytscha) |
Source/Tissue | Liver |
Morphology | Fibroblastoid |
Cell type | |
Growth mode | Adherent, monolayer |
Doubling time | ≈ 80 hours |
Life span | Infinite, continuous culture |
Karyotype | |
Transformed | |
Tumorigenic | |
Drug resistance | |
Biosafety Level | 1 REF |
Gene Expression | |
Products | |
Applications | |
Remarks | |
Related cell lines |
Cell line isolation information
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Recommended propagation method
Culture medium: 90% MEM (with Earle’s salts) + 10% heat inactivated FBS
Culture conditions: CO2, 20°C.
Split ratio: 1:2
Split frequency: Every 7-10 days
Harvesting time: Confluent culture
Split Method: Trypsin/EDTA
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ?
Availability
- DSMZ : Catalog No. : Product link
REFERENCES
- Ahne, 1985. Use of fish cell cultures for toxicity determination in order to reduce and replace the fish tests. Zentralbl Bakteriol Mikrobiol Hyg B. 180(5-6), 480-504. PMID-4024776
- DSMZ
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