- Cell Line: HCC1954 BL
- Synonyms:
- HCC-1954BL
- HCC1954-BL
- HCC1954BL
- Hamon Cancer Center 1954 BL
- Culture Type: Cell line
- Keywords: Epstein-Barr virus (EBV) transformed normal B lymphoblast cells
- Description: Established from the peripheral blood of a 61-year-old, Asian woman with ductal breast carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 61 years
- Ethnicity: Asian
- Gender: Female
- Tissue (origin): Peripheral blood
- Disease: Normal
- Disease subtype: Matched Normal (an autologous carcinoma cell line HCC1954 is available)
- Year of origin:-
- Organism:
- Cell line features:
- Morphology: Lymphoblast-like, Round to polymorphic cells
- Cell Type: B lymphoblast
- Growth mode: Suspension, single cells and multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Immortalization method: Epstein-Barr virus (EBV) transformed
- Gene expression:-
- Sequence variations (gene mutations/deletions/fusions/translocations):
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- Morphology: Lymphoblast-like, Round to polymorphic cells
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
- Medium Renewal: Every 2 to 3 days (depending on cell density)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Immunology
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- https://www.atcc.org/products/crl-2339
- https://www.cellosaurus.org/CVCL_1260
- https://depmap.org/portal/cell_line/ACH-002384?tab=overview
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full Text: Wiley (Download PDF)
- Images
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- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- EBV (Epstein-Barr virus)
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