Cell Line: LMSU

CELL LINE ORIGIN
OrganismHuman (Homo sapiens), 27 years old Japanese female
Source/TissueDerived from lymph node metastasis of gastric cancer
DiseaseGastric cancer, sarcoma
Year of origin 
CELL LINE FEATURES
MorphologyEpithelial-like
Cell type?
Growth modeAdherent, Monolayer
Doubling time?
Life spanInfinite (continuous culture)
Transformed?
Tumorigenic?
Drug resistance?
Products?
Karyotype?
  
GENE EXPRESSION
  
APPLICATIONS
 Cancer research
CULTURE MAINTENANCE AND STORAGE
Culture conditions and handling
Biosafety Level1 (It may differ in your country, follow the guidelines of your institute/country)
Plating efficiency?
Temperature37°C
AtmosphereHumidified incubator, 95% air + 5% CO2
Base mediumHamF10
Complete culture mediumHamF10 + 10% FBS
Medium RenewalEvery 2 to 3 days
Subculturing procedure
Harvesting timeSubconfluent culture
Split methodTrypsin or Trypsin/EDTA
Split ratio1:4 to 1:8
Seeding density 
Split frequency 
Cryopreservation
Cryomedium

Complete culture medium + 5% DMSO

OR

Complete culture medium + 10% DMSO

Storage conditions
Liquid nitrogen vapor phase
AVAILABILITY / SOURCE

American Type Culture Collection (ATCC)

 

Public Health England Culture Collections

 

Sigma-Aldrich

 

Japanese Collection of Research Bioresources (JCRB)

 

Cell Bank Australia

 

ACCEGEN BIOTECHNOLOGY

 

Abcam

 

ThermoFisher Scientific

 

rikenBRC

Product page link
 CELL LINE IDENTIFICATIONS
Synonyms
 
Remarks 
Related cell lines 

 

References

  • https://cellbank.brc.riken.jp/cell_bank/CellInfo/?cellNo=RCB1062

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