- Cell Line: NCI-H2081
- Synonyms:
- NCI-H2081
- NCIH2081
- H2081
- H-2081
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Cancer
- Description: Established from the lungs of a 59-year-old, Caucasian, female patient with small cell lung cancer, stage E
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 59 years
- Ethnicity: Caucasian
- Gender: Female
- Smoking habit: smoker
- Tissue (origin): Lung
- Disease: Small cell lung cancer, stage E
- Primary/Metastasis: Metastatic (Pleural effusion)
- Year of origin: 1988
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: floating aggregates of round cell clusters
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- PTEN (C124F mutation, Likely Oncogenic, Likely Loss-of-function)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000394?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000394?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium supplemented with
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite
- 10 nM Hydrocortisone
- 10 nM beta-estradiol
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time:-
- Seeding density:-
- Recommended split ratio:-
- Medium Renewal: every 3 to 4 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% (v/v) FBS + 10% (v/v) DMSO
- Complete growth medium + 5% (v/v) FBS + 10% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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