- Cell Line: NCI-H2347
- Synonyms:
- NCI-H2347
- NCIH2347
- H2347
- H-2347
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cell Line
- Description: Established from the lungs of a 54-year-old, Caucasian female patient with stage 1 non-small cell lung cancer (adenocarcinoma)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 54 years
- Ethnicity: Caucasian
- Gender: Female
- Smoking habit:-
- Tissue (origin): Lung
- Disease: Non-small cell lung cancer (adenocarcinoma), Stage 1
- Primary/Metastasis: Primary
- Year of origin: 1990
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: 38.6 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- KRAS (L19F mutation, Oncogenic, Gain-of-function, Heterozygous)
- NRAS (Q61R mutation, Oncogenic, Gain-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000875?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000875?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:2 to 1:6
- Medium Renewal: Every 2-3 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% (v/v) DMSO
- Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images: NCI-H2347 cell Image link (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
