- Cell Line: NCI-H460
- Synonyms:
- NCI-H460
- NCIH460
- H460
- H-460
- Culture Type: Cell line
- Keywords: Human Large Cell Lung Carcinoma Epithelial Cell Line
- Description: Established from the Pleural effusion (metastatic site) of a Caucasian, male patient with Large Cell Lung Carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion (metastatic site)
- Disease:
- Non-Small Cell Lung Cancer
- Disease subtype: Large Cell Lung Carcinoma
- Molecular subtype: NSCLC Large Cell
- Primary/Metastasis: Metastasis
- Non-Small Cell Lung Cancer
- Year of origin: 1982
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Hypotriploid, Modal number = 57; range = 53 to 65
- Doubling time: ~23 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity: Yes, in nude mice
- Gene expression:
- p53 mRNA is easily detectable at levels comparable to normal lung tissue
- Positive for keratin
- Positive for vimentin
- Negative for neurofilament triplet protein
- Sequence variations:
- KRAS (Q61H mutation, Oncogenic Gain-of-function, Homozygous)
- PIK3CA (E545K mutation, Oncogenic, Gain-of-function, Heterozygous)
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- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:8
- Medium Renewal: Two to three times weekly
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- High-throughput screening
- Toxicology
- Availability
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- Cyton: Product number: Product link
- AcceGen: Cat.No Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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