- Cell Line: NCI-H524
- Synonyms:
- NCI-H524
- NCIH524
- H524
- H-524
- Culture Type: Cell line
- Keywords: Human Lung Carcinoma Cell Line
- Description: Established from the lungs of a 63-year-old, Caucasian male with Stage L carcinoma
- Origin:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype:
- Doubling time: 100 hours (Cellosaurus)
- Microsatellite instability: Instable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- ATM (R337C mutation, Likely Oncogenic, Likely Loss-of-function, Heterozygous)
- TP53 (T155N mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000816?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000816?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time:-
- Seeding density:-
- Recommended split ratio:-
- Medium Renewal:-
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- References
- Images:
- NCI-H524 cell line image (Link: ATCC)
- NCI-H524 cell line image 1 (Link: Cytion)
- NCI-H524 cell line image 2 (Link: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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