- Cell Line: NCI-H650
- Synonyms:
- NCI-H650
- NCIH650
- H650
- H-650
- Culture Type: Cell line
- Keywords: Human Bronchioalveolar Carcinoma Cell Line
- Description: Established from the lungs of a Caucasian male patient with bronchioalveolar carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit: non-smoker
- Tissue (origin): Lung
- Disease: bronchioalveolar carcinoma (Non-Small Cell Lung Cancer)
- Primary/Metastasis: Metastatic (DepMap)
- Collection site: Lung
- Year of origin: 1983
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Spherical
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 90; range = 54 to 178; del(p14-p23), inv(3p)
- Doubling time: 49.4 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- KRAS (Q61L mutation, Oncogenic, Gain-of-function, Homozygous)
- TP53 (K164N mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000945?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000945?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 5% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time:-
- Seeding density:-
- Recommended split ratio:-
- Medium Renewal:-
- Cryopreservation:
- Freezing medium:
- Complete growth medium +7.5% DMSO
- Complete growth medium +7.5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images: NCI-H650 cell line image link (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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