- Cell Line: NK-92
- Synonyms:
- Culture Type: Cell line
- Keywords: Human Natural Killer Lymphoma Cell Line
- Description: Established from peripheral blood mononuclear cells from a 50-year-old, Caucasian male with rapidly progressive non-Hodgkin’s lymphoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Ethnicity: Caucasian
- Age: 50 years
- Gender: Male
- Species: Human (Homo sapiens)
- Tissue: Peripheral blood
- Disease: Malignant Non Hodgkins Lymphoma
- Primary/Metastasis:-
- Collection site:-
- Year of origin: 1992
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type: Natural killer cell
- Morphology: lymphoblast-like
- Growth mode: Suspension, cells grow in clumps
- Life span: Infinite (continuous culture)
- Doubling time: ca. 40-50 hours (dsmz)
- Karyotype: Human hypotetraploid karyotype with 4% polyploidy
- Microsatellite instability:-
- Special features: Cells are cytotoxic to a wide range of malignant cells such as K562 and Daudi cells
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:-
- Cell Type: Natural killer cell
- Handling and maintenance:
- Biosafety level:
- Complete culture medium: MyeloCult™ H5100 (StemCell Technologies cat # 05150) + Supplements
- Add the following supplement per 500 ml of MyeloCult™ H5100 medium
- 63 mL Gibco™ Horse Serum (New Zealand origin)
- 10 μg IL-2 IS: Use 1 mL/500 mL culture media
- For more information, please visit ATCC
- 63 mL Gibco™ Horse Serum (New Zealand origin)
- Add the following supplement per 500 ml of MyeloCult™ H5100 medium
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time:-
- Seeding density: 2 x 105 to 3 x 105 viable cells/mL (These cells tend to grow in aggregates that may lose viability when they are dispersed. Accurate counts and viabilities may not be possible.)
- Split ratio: 1:2
- Split frequency:-
- Medium Renewal: every 2 to 3 days (cells are extremely sensitive to overgrowth and media exhaustion)
- Cryopreservation:
- Freezing medium: CryoStor® CS10 (StemCell Technologies at # 07930) (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: CryoStor® CS10 (StemCell Technologies at # 07930) (ATCC)
- Applications:
- Immunology
- 3D cell culture
- Toxicology
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- NK-92 cell line image link 1 (Source: ATCC)
- NK-92 cell line image link 2 (Source: DSMZ)
- NK-92 cell line image link 3 (Source: DSMZ)
- NK-92 cell line image link 4 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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