- Cell Line: NU-DUL-1
- Synonyms:
- Culture Type: Cell line
- Keywords: Human B cell lymphoma cell line
- Description: Established from the peritoneal effusion of a 43-year-old, Caucasian male with non-Burkitt’s lymphoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 43 years
- Ethnicity: Caucasian
- Gender: Male
- Species: Human (Homo sapiens)
- Tissue (origin): Peritoneal effusion
- Disease: non-Burkitt’s lymphoma
- Year of origin: 1985
- Organism:
- Cell line features:
- Cell Type: lymphocyte
- Morphology: single, round to polymorph cells
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: hypodiploid
- Doubling time: ~ 30-36 hour
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- Cell Type: lymphocyte
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% (v/v) FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 8.0 x 105 and 1.0 x 106 cells/mL
- Seeding density: 2.0 x 105 to 3.0 x 105 cells/mL
- Recommended split ratio: 1:2 to 1:5
- Medium Renewal: every 2 to 3 days
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Availability:
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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