Cell Line: NU-DUL-1

  • Cell Line: NU-DUL-1
  • Synonyms:
  • Culture Type: Cell line
  • Keywords: Human B cell lymphoma cell line
  • Description: Established from the peritoneal effusion of a 43-year-old, Caucasian male with non-Burkitt’s lymphoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 43 years
      • Ethnicity: Caucasian
      • Gender: Male
    • Tissue (origin): Peritoneal effusion
    • Disease: non-Burkitt’s lymphoma
    • Year of origin: 1985
  • Cell line features:
    • Cell Type: lymphocyte
    • Morphology: single, round to polymorph cells
    • Growth mode: Suspension
    • Life span: Infinite (continuous culture)
    • Karyotype: hypodiploid
    • Doubling time: ~ 30-36 hour
    • Special features:-
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:
    • Sequence variations:
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: RPMI-1640 Medium + 10% (v/v) FBS
    • Culture conditions: 
      • Temperature: Humidified 37°C incubator
      • Atmosphere: 5% CO2, 95% Air
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: 8.0 x 105 and 1.0 x 106 cells/mL
      • Seeding density: 2.0 x 105 to 3.0 x 105 cells/mL
      • Recommended split ratio: 1:2 to 1:5
      • Medium Renewal: every 2 to 3 days
    • Cryopreservation:
      • Freezing medium:
        • Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
  • Availability:
    • DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
    • American Type Culture Collection (ATCC): Product link

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