| OVERVIEW | |
| Organism | Human (Homo sapiens), 52-year-old white male |
| Source/Tissue of origin | Liver, Ascites Established from Adenocarcinoma |
| Year of isolation | 1971 |
| Morphology | Epithelial-like |
| Cell type | Endothelial |
| Growth mode | Adherent |
| Doubling time | 30 hours |
| Life span | Infinite, continuous culture |
| Karyotype | Hypotriploid |
| Transformed | |
| Tumorigenic | Yes (in nude mice) |
| Drug resistance | ? |
| Gene Expression | |
| Positive for |
|
| Altered gene | |
| Enzymes | |
| Products | |
| Applications | 3D cell culture Cardiovascular disease research Cancer research High-throughput screening Toxicology |
| Remarks | |
| Keywords | Human liver cancer cell line |
| Related cell lines | |
| Culture Maintenance | |
| Biosafety Level | BSL 1 (Ref: ATCC) (It may differ in your country, follow the guidelines of your institute/country) |
| Culture medium | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate + 10% FBS |
| Culture conditions | Humidified incubator, 37°C, 5% CO2 |
| Subculturing procedure | Trypsin-EDTA |
| Culture storage | |
| Cryo-medium | Complete growth medium + 5% (v/v) (Ref: ATCC) |
| Storage conditions | Vapor phase of liquid nitrogen |
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