- Cell Line: SW-48
- Synonyms:
- SW-48
- SW 48
- SW48
- Culture Type: Cell line
- Keywords: Human Colon Adenocarcinoma Cell Line
- Description: Established from a 82 year old Caucasian female patient with colorectal adenocarcinoma (Dukes’ type C, grade IV)
- Origin:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:
- 42.33 hours (Cellosaurus)
- 33 hours (Cellosaurus)
- 42.33 hours (Cellosaurus)
- Microsatellite instability: Instable (Cellosaurus)
- Special features: positive for keratin by immunoperoxidase staining
- Product: Carcinoembryonic antigen (CEA)
- Soft agar colony formation assay:-
- Tumorigenicity: tumorigenic in nude mice
- Gene expression:
- Sequence variations:
- MAP2K1 (Q56P mutation, Oncogenic, Gain-of-function) (DepMap)
- MAP2K1 (H119Y mutation, Likely Oncogenic, Likely Gain-of-function) (DepMap)
- CTNNB1 (S33Y mutation, Oncogenic, Gain-of-function) (DepMap)
- PIK3CA (G914R mutation, Likely Oncogenic, Likely Gain-of-function) (DepMap)
- MED12 (R18W mutation, Oncogenic, Likely Loss-of-function) (DepMap)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000958?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000958?tab=fusions
- MAP2K1 (Q56P mutation, Oncogenic, Gain-of-function) (DepMap)
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:2 to 1:6
- Medium Renewal: 1 to 2 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- Cancer research
- Transfection host
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- Cytion: Product link
- SigmaAldrich: Product link
- References
- Images:
- SW-48 cell line image 1 (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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