- Cell Line: THP-1 GAS-Luc2
- Synonyms:
- THP1 GAS-Luc2
- THP 1 GAS-Luc2
- THP1 GAS-Luc2
- Culture Type: Cell line
- Keywords: Human acute monocytic leukemia cell line
- Description:
- Parent cell line: THP-1
- THP-1 cells stably express firefly luciferase gene (luc2) under control of a gamma-activated site (GAS) promoter
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 1 year
- Ethnicity:-
- Gender: Male
- Tissue: Peripheral blood
- Disease:
- Acantholytic Acute monocytic leukemia
- Molecular subtype: M5 (depmap)
- Acantholytic Acute monocytic leukemia
- Year of origin: 2020
- Organism:
- Cell line features:
- Cell Type: Monocyte
- Morphology: large, round
- Growth mode: single cells in suspension, partly in clusters
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: ≈ 72 hrs (ATCC)
- Gene expression:-
- Sequence variations:-
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 1 µg/mL Puromycin + 0.05 mM 2-Mercaptoethanol + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Maintain culture between 0.2 x 106 cells/ml to 1.0 x 106 cells/ml
- Harvesting time: When culture density reaches 8 x105 cells/mL. Do not allow the cell density to exceed 1 x 106 cells/mL. (ATCC)
- Split ratio:-
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 6 – 8 x 106 cells/ml
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Reporter assay
- Assay development
- Immunology
- Immuno-oncology
- Toxicology
- Cell and gene therapy (CGT) development
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- ATCC
- Cellosaurus
- Tsuchiya et al., 1980. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 26(2), 171-176. PMID-6970727; Full Text: Wiley
- Brasier et al., 1989. Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines. Biotechniques 7(10): 1116-22, 1989. PMID-2698191
- Grewal et al., 2001. Identification of a γ-interferon-responsive element in the promoter of the human macrophage scavenger receptor A gene. Arterioscler Thromb Vasc Biol 21, 825-31. PMID-11348881; Full Text: ahajournals (Download PDF)
- Tsukada et al., 1996. A novel STAT-like factor mediates lipopolysaccharides, interleukin 1 (IL-1), and IL-6 signaling and recognizes a gamma interferon activation site-like element in the IL1B gene. Mol Cell Biol 16(5): 2183-94. PMID-8628285; Full Text: tandfonline, PMC (Download PDF)
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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