Cell Line : U3123MG

Overview 
OrganismHuman (Homo sapiens), 64 years old, Male
Source/TissueGlioblastoma
Morphology 
Cell typeGlioma stem cell / Glioblastoma stem cells
Growth modeAdherent / Sphere
Doubling timeLow proliferative capacity
Life spanInfinite
Karyotype?
TransformedNaturally transformed
TumorigenicYes (immunocompromised mice)
Drug resistance?
Biosafety Level?
Gene Expression?
Products?
ApplicationsGlioma research
Remarks?
Related cell lines 

CELL LINE ISOLATION INFORMATION

The cell line U3123MG was established from a Glioblastoma tumor biopsy taken from a 64 years old male patient.

Dissociated cells from tumor tissue was allowed to grow as spheres onto uncoated dishes in complete culture medium [1:1 mix of Neurobasal and DMEM/F12 media supplemented with N2 (1x final conc), B27 (1x final conc), human recombinant FGF2 (final conc. 10 ng/ml) and human EGF (final conc. 10 ng/ml)]. These neurospheres were plated onto laminin coated dishes after 5 to 7 days to allow them to grow as adherent culture. Please read Xie et al., 2015 supplementary data for exact details.

Recommended propagation method

Culture medium: Neurobasal and DMEM/F12 media (1:1 mix) containing N2 and B27 supplements and human recombinant FGF2 and EGF (10 ng/ml)
Culture dishes: Primaria dishes coated with mouse laminin 

Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C
Split ratio: 1:4 to 1:6
Split frequency: ?
Harvesting time: Subconfluent culture
Split Method: TrypLE

Recommended Cryopreservation Method

Freeze medium: Culture medium (complete medium containing all ingredients including FGF2 and EGF) supplemented with 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ≈ 5 x 105 cells/ml

Availability

REFERENCES

  • Xie et al., 2015. The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes. EBioMedicine. 2(10), 1351-63. PMID-26629530; Full Text Link: thelancet, PMC

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