ExpSup: Pragmin Tyrosine Phosphorylation at EPIYA Motif is Required for Morphological Changes in AGS Cells

Note: For more detailed and accurate information, please read the cited reference.

• Pragmin overexpression induces morphological changes in AGS cells, while mutant Pragmin (Y391F mutation at the EPIYA motif) does not. (Safari et al., 2011) 
  • Experiment details: 
    • Cell line: AGS cell line transiently expressing Myc-tagged Pragmin/Myc-tagged mutant Pragmin (Y391F mutation at the EPIYA motif) (Rat cDNA)
      
    • Method: Phase contrast microscopy.

• Explanation: 
  • Changes in morphology was associated with SFK activation (Safari et al., 2011)

References:

• Safari et al., 2011. Mammalian Pragmin regulates Src family kinases via the Glu-Pro-Ile-Tyr-Ala (EPIYA) motif that is exploited by bacterial effectors. Proc Natl Acad Sci USA. 108(36), 14938-14943. PMID-21873224; Full Text: pnas,  PMC