- Cell Line: NCI-H522
- Synonyms:
- NCIH522
- NCI-H522
- H522
- H-522
- Culture Type: Cell line
- Keywords: Human Non-Small Cell Lung Adenocarcinoma Epithelial Cell Line
- Description: Established from the primary tumor of a 58-year-old White male with Non-Small Cell Lung Adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 58 years
- Ethnicity: White
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lung
- Disease:
- Lung cancer
- Molecular subtype: Non-Small Cell Lung Adenocarcinoma
- Primary/Metastasis: Primary
- Lung cancer
- Year of origin:
- Organism:
- Cell line features:
- Cell Type: Epithelial cell
- Morphology: Epithelial-like
- Growth mode: Adherent, single cells and loosely attached clusters
- Life span: Infinite (continuous culture)
- Karyotype: hypotriploid, Modal number = 53
- Doubling time:
- 20 hours (Cellosaurus)
- 48.1 hours (Cellosaurus)
- 20 hours (Cellosaurus)
- Microsatellite instability: Stable
- Special features:
- Product:-
- Tumorigenicity:-
- Gene expression:
- Positive for keratin
- Positive for vimentin
- Negative for neurofilament triplet protein
- Sequence variations:
- Cell Type: Epithelial cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Mechanical, Shaking
- Harvesting time:-
- Split ratio:-
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Mechanical, Shaking
- Cryopreservation:
- Freezing medium: Complete growth medium + 50% FBS + 10% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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