- Cell Line: NCI-H660
- Synonyms:
- NCIH660
- NCI-H660
- H660
- H-660
- Culture Type: Cell line
- Keywords: Human Prostate Small Cell Carcinoma Epithelial Neuroendocrine Cell Line
- Description: Established from the metastatic tumor (lymph node) of a White, 63-year-old, male patient with carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 63 years
- Ethnicity: White
- Gender: Male
- Tissue (origin): Prostate
- Tissue (isolation): Lymph node (Metastatic site)
- Disease:
- Prostate Small Cell Carcinoma
- Primary/Metastasis: Metastatic
- Prostate Small Cell Carcinoma
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Epithelial neuroendocrine cell
- Morphology: Epithelial-like
- Growth mode: Mixed: floating aggregates of round cells with some attached cells
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: ≈ 100 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:
- Express functional atrial natriuretic peptide (ANP) receptors
- Sequence variations:
- Cell Type: Epithelial neuroendocrine cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 0.005 mg/ml Insulin + 0.01 mg/ml Transferrin + 30nM Sodium selenite (final conc.) + 10 nM Hydrocortisone (final conc.) + 10 nM beta-estradiol (final conc.) + extra 2mM L-glutamine (for final conc. of 4 mM) + 5% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Mechanical, Shaking
- Harvesting time:-
- Split ratio:-
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Mechanical, Shaking
- Cryopreservation:
- Freezing medium: Complete growth medium + 50% FBS + 10% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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