- Cell Line: NCI-H522
- Synonyms:
- NCIH522
- NCI-H522
- H522
- H-522
- Culture Type: Cell line
- Keywords: Human Non-Small Cell Lung Adenocarcinoma Epithelial Cell Line
- Description: Established from the primary tumor of a 58-year-old White male with Non-Small Cell Lung Adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 58 years
- Ethnicity: White
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lung
- Disease:
- Lung cancer
- Molecular subtype: Non-Small Cell Lung Adenocarcinoma
- Primary/Metastasis: Primary
- Lung cancer
- Year of origin:
- Organism:
- Cell line features:
- Cell Type: Epithelial cell
- Morphology: Epithelial-like
- Growth mode: Adherent, single cells and loosely attached clusters
- Life span: Infinite (continuous culture)
- Karyotype: hypotriploid, Modal number = 53
- Doubling time:
- 20 hours (Cellosaurus)
- 48.1 hours (Cellosaurus)
- 20 hours (Cellosaurus)
- Microsatellite instability: Stable
- Special features:
- Product:-
- Tumorigenicity:-
- Gene expression:
- Positive for keratin
- Positive for vimentin
- Negative for neurofilament triplet protein
- Sequence variations:
- Cell Type: Epithelial cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Mechanical, Shaking
- Harvesting time:-
- Split ratio:-
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Mechanical, Shaking
- Cryopreservation:
- Freezing medium: Complete growth medium + 50% FBS + 10% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
