- Cell Line: NCI-H345
- Synonyms:
- NCI-H345
- NCIH345
- H345
- H-345
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell line
- Description: Established from bone marrow metastatic tumor of a 40-year-old, Caucasian, male with small cell lung Carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:
- 40 years (ATCC)
- 64 Years (Cellosaurus)
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Bone marrow
- Disease:
- Small cell lung carcinoma
- Primary/Metastasis: Metastatic
- Year of origin: 1981
- Organism:
- Cell line features:
- Cell Type:
- Morphology: Epithelial (ATCC)
- Growth mode: Mixed: multicellular aggregates in suspension and some adherent cells
- Life span: Infinite (continuous culture)
- Karyotype: Hypotriploid, Modal number = 63, Range = 60 to 66
Doubling time:- - Microsatellite instability: Stable
- Special features:
- Product: Gastrin releasing peptide
- Tumorigenicity: Produce tumors in nude mice
- Gene expression:
- Expression of biochemical markers of SCLC:
- neuron specific enolase
- brain isoenzyme of creatine kinase
- L-DOPA decarboxylase
- bombesin-like immunoreactivity
- Expression of biochemical markers of SCLC:
- Sequence variations:
- TP53 (Y236C mutation, Likely Oncogenic, Loss-of-function, Homozygous)
- For information, please visit
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: HITES medium (serum-free)
- Base medium: DMEM: F-12 Medium (ATCC 30-2006) + Supplements
- Supplements: Insulin 0.005mg/ml (Gibco 12585-014) + Transferrin 0.01 mg/ml (Sigma T5391 or equivalent) + Sodium selenite 30 nM (Sigma S9133 or equivalent) + Hydrocortisone 10 nM (Sigma H0135 or equivalent) + beta-estradiol 10 nM (Sigma E2257 or equivalent) + L-Glutamine Solution (ATCC 30-2214) 2 mM (in addition to that in the base medium) (See ATCC)
- Base medium: DMEM: F-12 Medium (ATCC 30-2006) + Supplements
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (Addition or replacement of medium)
- Harvesting time:-
- Seeding density:-
- Split ratio:-
- Medium Renewal: Every 3 to 4 days (depending on cell density)
- Subculturing method: Dilution (Addition or replacement of medium)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 7.5% DMSO
- Complete growth medium + 7.5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Immunology
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- SCLC (Small Cell Lung Carcinoma)
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