- Cell Line: NCI-H146
- Synonyms:
- NCI-H146
- NCIH146
- H146
- H-146
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell line
- Description: Established from bone marrow metastatic tumor of a 59-year-old, Caucasian, male with small cell lung carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 59 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Bone marrow
- Disease:
- Small cell lung carcinoma
- Primary/Metastasis: Metastatic
- Year of origin: 1979
- Organism:
- Cell line features:
- Cell Type:
- Morphology:
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype: Near triploid, Modal chromosome number: 68
Doubling time:- - Microsatellite instability: Stable
- Special features:
- Product: Insulin-like growth factor II (IGF II)
- Tumorigenicity: Produce tumors in nude mice
- Gene expression:
- Oncogene expression:
- myc positive
- myb positive
- raf positive
- ras positive
- fms positive
- fes negative
- Expression of biochemical markers:
- neuron specific enolase
- brain isoenzyme of creatine kinase
- L-DOPA decarboxylase
- bombesin-like immunoreactivity
- Other gene expression
- Vasopressin negative
- oxytocin negative
- gastrin releasing peptide negative
- vimentin positive by immunostaining
- keratin positive by immunostaining
- neurofilament triplet protein negative
- produce relatively high amounts of c-myc mRNA (no amplification of c-myc DNA sequences)
- Oncogene expression:
- Sequence variations:
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (Addition or replacement of medium)
- Harvesting time:-
- Seeding density:-
- Split ratio: 1:2 to 1:6
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Dilution (Addition or replacement of medium)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Immunology
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- AcceGen: Cat.No Product link
- Korean Cell Line Bank: Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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