- Cell Line: NCI-H747
- Synonyms:
- NCI-H747
- NCIH747
- H747
- H-747
- Culture Type: Cell line
- Keywords: Human Colorectal Adenocarcinoma Cell line
- Description: Established from the common duct node metastasis of a Caucasian, 69-year-old, male patient with colorectal adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 69 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Large intestine, Cecum
- Tissue (isolation): Common duct node (ATCC)/Lymph node (Cellosaurus)
- Disease:
- Colorectal Adenocarcinoma
- Primary/Metastasis: Metastatic
- Year of origin:
- Organism:
- Cell line features:
- Cell Type:
- Morphology: Epithelial (ATCC)
- Growth mode: Adherent, floating aggregates of round cells with some attached cells
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 66, Range = 63 to 75
Doubling time:- - Microsatellite instability:
- Stable (Cellosaurus)
- Instable (Cellosaurus)
- Special features:
- Product: Carcinoembryonic antigen (CEA)
- Tumorigenicity: Produce tumors in nude mice
- Gene expression:
- Antigen
- Negative for Dopa decarboxylase
- Negative for TAG-72 antigen
- Positive for CA19-9 antigen
- Antigen
- Sequence variations:
- KRAS (G13D mutation, Oncogenic Gain-of-function, Heterozygous)
- TP53 (R158L mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation + harvesting floating cells by centrifugation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Split ratio: 1:2 to 1:4
- Medium Renewal: Twice per week
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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