- Cell Line: NCI-BL1395
- Synonyms:
- NCI-BL1395
- NCIBL1395
- Culture Type: Cell line
- Keywords: Human B lymphoblasts EBV transformed cell line
- Description: Established from B lymphoblasts, isolated from the peripheral blood of a normal 55-year-old, Caucasian female donor, EBV transformed
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 55 years
- Ethnicity: Caucasian
- Gender: Female
- Tissue (origin): Peripheral blood
- Disease: Normal
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: B lymphoblast
- Morphology: lymphoblast-like
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time: – - Microsatellite instability:-
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- ATM (T2666A mutation, Likely Oncogenic, Likely Loss-of-function)
- PTPRT (Q479E mutation,, Likely Oncogenic, Likely Loss-of-function)
- BRAF (G469A mutation, Oncogenic, Gain-of-function) (DepMap)
- Cell Type: B lymphoblast
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL)
- Cell dissociation solution: NA
- Harvesting time: 1 x 106 viable cells/mL
- Seeding density: 2 to 3 x 105 viable cells/mL
- Recommended split ratio:-
- Medium Renewal: Add fresh medium every 2 to 3 days
- Subculturing method: Dilution (Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Immunology
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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