Cell Line: NCI-H1755

  • Cell Line: NCI-H1755
  • Synonyms:
    • NCI-H1755
    • NCIH1755
    • H1755
    • H-1755
  • Culture Type: Cell line
  • Keywords: Human Lung Adenocarcinoma Cell line
  • Description: Established from the metastatic tumor (Liver) of a 65-year-old caucasian female with non-small cell lung cancer
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 65 years
      • Ethnicity: Caucasian
      • Gender: Female
      • Smoking habit: smoker
    • Tissue (origin): Lung
    • Disease: Non-small cell lung cancer
      • Disease subtype: Lung Adenocarcinoma
      • Molecular subtype:-
    • Primary/Metastasis: Metastatic (Liver)
    • Year of origin: 1987
  • Cell line features:
    • Cell Type:-
    • Morphology: Epithelial-like and/or rounded
    • Growth mode: Mixed: Adherent and small clusters in suspension 
    • Life span: Infinite (continuous culture)
    • Karyotype:-
      Doubling time: –
    • Microsatellite instability: Stable
    • Special features:
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:
    • Sequence variations:-
  • Handling and maintenance:-
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: RPMI-1640 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: Humidified 37°C incubator
      • Atmosphere: 5% CO2, 95% Air
    • Subculturing:
      • Subculturing method: Dilution (suspension cells) and Enzymatic dissociation (attached cells) using 0.25% (w/v) Trypsin-0.53 mM EDTA solution
      • Cell dissociation solution:
      • Harvesting time:-
      • Seeding density:-
      • Recommended split ratio: 1:3 to 1:10
      • Medium Renewal: Every 3 to 4 days
    • Cryopreservation:
      • Freezing medium:
        • Complete growth medium +  40% (v/v) FBS + 5% (v/v) DMSO
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • Cancer research
    • 3D cell culture
  • Availability:
  • References
  • Images:-
  • Abbreviations
    • BSL (Biosafety level)
    • FBS (Fetal Bovine Serum)
    • DMSO (Dimethyl sulfoxide)
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