- Cell Line: NCI-BL1395
- Synonyms:
- NCI-BL1395
- NCIBL1395
- Culture Type: Cell line
- Keywords: Human B lymphoblasts EBV transformed cell line
- Description: Established from B lymphoblasts, isolated from the peripheral blood of a normal 55-year-old, Caucasian female donor, EBV transformed
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 55 years
- Ethnicity: Caucasian
- Gender: Female
- Tissue (origin): Peripheral blood
- Disease: Normal
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: B lymphoblast
- Morphology: lymphoblast-like
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time: – - Microsatellite instability:-
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- ATM (T2666A mutation, Likely Oncogenic, Likely Loss-of-function)
- PTPRT (Q479E mutation,, Likely Oncogenic, Likely Loss-of-function)
- BRAF (G469A mutation, Oncogenic, Gain-of-function) (DepMap)
- Cell Type: B lymphoblast
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL)
- Cell dissociation solution: NA
- Harvesting time: 1 x 106 viable cells/mL
- Seeding density: 2 to 3 x 105 viable cells/mL
- Recommended split ratio:-
- Medium Renewal: Add fresh medium every 2 to 3 days
- Subculturing method: Dilution (Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Immunology
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
Was this post helpful?
Let us know if you liked the post. That’s the only way we can improve.
