- Cell Line: SW-1710
- Synonyms:
- SW-1710
- SW 1710
- SW1710
- Culture Type: Cell line
- Keywords: Human Urinary Bladder Transitional Cell Carcinoma Cell Line
- Description: Established from bladder tumor of an 84-year-old Caucasian woman following transurethral tumor resection in 1977
- Origin:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like/Elongated cells
- Growth mode: Adherent, Monolayer
- Life span: Infinite (continuous culture)
- Karyotype: Human flat-moded hypotetraploid karyotype with 8% polyploidy
- Doubling time: ~25-32 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- TP53 (R273C mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000566?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000566?tab=fusions
- TP53 (R273C mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- DMEM + 15% heat-inactivated FBS (DSMZ)
- DMEM + 15% heat-inactivated FBS (DSMZ)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin/EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:5 to 1:10
- Medium Renewal: 2 to 3 times per week?
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (DSMZ)
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (DSMZ)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 1-1.5 x 106 cells/ml
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- Cancer research
- Availability:
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- SW-1710 cell line image link 1 (Source: DSMZ)
- SW-1710 cell line image link 2 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Dulbecco’s Modified Eagle Medium (DMEM)
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