- Cell Line: SW-1222
- Synonyms:
- SW-1222
- SW 1222
- Culture Type: Cell line
- Keywords: Human Colorectal Adenocarcinoma Cell Line
- Description: Established from a patient with a moderately well differentiated adenocarcinoma of the colon classified as Dukes’ stage C
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity:-
- Gender: female
- Tissue: Large intestine, Colon
- Disease: Colorectal adenocarcinoma (Dukes’ type C)
- Primary/Metastasis: Primary
- Collection site: Colon
- Year of origin:-
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent, Sometimes aggregated in small groups
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: produce tumours in female athymic nu/nu nude mice
- Gene expression:-
- Sequence variations:
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- Iscove’s Modified Dulbecco’s Medium + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
- Iscove’s Modified Dulbecco’s Medium + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin- EDTA solution
- Harvesting time: Subconfluent culture (70% confluency) (Cells do not reach confluency but will grow in small islands)
- Seeding density:-
- Recommended split ratio: 1:2 to 1:5
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 1×106 cells/ml
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- Drug Discovery & Development
- Availability:
- UK Health Security Agency Culture Collections: Product link
- Sigma Aldrich: Product link
- CancerTools.org: Product link
- Ximbio: Product link
- References
- Images:
- SW-1222 cell line image link 1 (Source: ximbio)
- SW-1222 cell line image link 2 (Source: Cancertools)
- SW-122 cell line image link 3 (Source: Cellosaurus)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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