- Cell Line: SV-T2
- Synonyms:
- BALB 3T3 SV-T2
- SVT2
- Culture Type: Cell line
- Keywords: Mouse Embryo Transformed Cell Line
- Description: Derived by SV40 transformation of the line BALB/3T3 clone A31
- Origin:
- Organism:
- Species: Mouse (Mus musculus)
- Breed/subspecies/Strain/Ethnicity: BALB/c
- Age: 14 to 17 days gestation
- Gender:-
- Tissue: Embryo
- Disease:-
- Primary/Metastasis:-
- Collection site: Embryo
- Year of origin:-
- Country of Origin: United States
- Organism:
- Cell line features:
- Cell Type: Fibroblast
- Morphology: Fibroblast-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Doubling time: –
- Karyotype:-
- Microsatellite instability:-
- Special features: SV40 transformed
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: Yes, in BALB/c mice
- Gene expression:-
- Sequence variations:
- Cell Type: Fibroblast
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Dulbecco’s modified Eagle’s medium + 10% bovine calf serum
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.1% trypsin solution
- Harvesting time: Sub-confluent cultures (~80%)
- Seeding density:-
- Split ratio: 1:4 to 1:10
- Split frequency: Twice per week
- Medium Renewal:-
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Applications:
- 3D cell culture
- tumourigenicity and virus studies
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- Japanese Collection of Research Bioresources (JCRB): Product link
- Public Health England Culture Collections: Product link
- Sigma-Aldrich: Product link
- AcceGen™: Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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