Cell Line: SK-MEL-1

  • Cell Line: SK-MEL-1
  • Synonyms:
    • SK-MEL-1
    • SKMEL1
    • SkMEL-1
    • SK-Mel-1
    • SK Mel 1
    • SK-Mel1
  • Culture Type: Cell line
  • Keywords:  Human Melanoma Cell Line
  • Description: Established from the lymph node metastasis (thoracic duct) of a 29-year-old Caucasian male with primary melanoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 29 years
      • Ethnicity: Caucasian
      • Gender: Male
    • Tissue: Skin
    • Disease: Melanoma
    • Primary/Metastasis: Metastatic (DepMap)
    • Collection site: Thoracic lymph duct
    • Year of origin: 1966
    • Country of Origin:-
  • Cell line features:
    • Cell Type:
    • Morphology: Mostly round cells growing singly or in clumps
    • Growth mode: Suspension
    • Life span: Infinite (continuous culture)
    • Doubling time: ~100 hours  (DSMZ)
    • Karyotype: Human hypertriploid karyotype with 4% polyploid
    • Microsatellite instability: Stable
    • Special features:-
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:
      • Yes, in nude mice
      • pigment-producing tumors upon heterotransplantation into hamster (DSMZ)
    • Gene expression:-
    • Sequence variations:
      • CTNNB1 (S33C mutation, Oncogenic, Gain-of-function, Heterozygous)
      • BRAF (V600E mutation, Oncogenic, Gain-of-function, Heterozygous)
      • For more information, please visit
        • https://depmap.org/portal/cell_line/ACH-000465?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000465?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (DSMZ, ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium:
      • Eagle’s Minimum Essential Medium + 10% FBS (ATCC)
      • RPMI 1640 + 10-20% heat-inactivated FBS (DSMZ)
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: 0.5-1.0 x 106 cells/ml
      • Seeding density:  1 x 105 cells/mL
      • Split ratio:-
      • Split frequency:-
      • Medium Renewal: 2 to 3 times per week
    • Cryopreservation:
      • Freezing medium:
        • Complete growth medium + 5 % (v/v) DMSO (ATCC)
        • Complete growth medium + 20 % (v/v) FBS + 10 % (v/v) DMSO (DSMZ)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Cancer research
  • Cell Line Availability: The cell line can be obtained from the following sources.

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