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Cell Line: SK-MM-2

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  • Cell Line: SK-MM-2
  • Synonyms:
    • SK-MM-2
    • SKMM-2
    • SK-MM2
    • SKMM2
  • Culture Type: Cell line
  • Keywords:  Human Plasma Cell Leukemia Cell Line
  • Description: Established from the peripheral blood of a 54-year-old Caucasian male patient with plasma cell leukemia (Igkappa) (refractory, terminal state)
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 54 years
      • Ethnicity: Caucasian
      • Gender: Male
    • Tissue: Lymphoid (DepMap)
    • Disease: Plasma cell leukemia (Igkappa) (refractory, terminal state)
    • Primary/Metastasis: Primary (DepMap)
    • Collection site: Peripheral blood
    • Year of origin: 1982
    • Country of Origin:-
  • Cell line features:
    • Cell Type: B-cell
    • Morphology: Round single cells
    • Growth mode: Suspension
    • Life span: Infinite (continuous culture)
    • Doubling time:  
    • Karyotype: Human bimodal hypodiploid/hypotetraploid karyotype with 9% polyploidy
    • Microsatellite instability: Stable
    • Special features:-
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:-
    • Sequence variations:
      • TP53 (K132N mutation, Likely Oncogenic, Likely Loss-of-function)
      • For more information, please visit
        • https://depmap.org/portal/cell_line/ACH-000363?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000363?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: RPMI 1640 + 20% heat-inactivated FBS
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: Saturated culture (DSMZ)
      • Seeding density:  1.0 x 106 cells/ml (DSMZ)
      • Split ratio: 1:2 (DSMZ)
      • Split frequency: every 4-5 days (DSMZ)
      • Medium Renewal:-
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 20 % (v/v) FBS + 10 % (v/v) DMSO (DSMZ)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density): 5 x 106 cells/ml
      • Volume per vial: 1 ml
  • Applications: 
    • Immunology
  • Cell Line Availability: The cell line can be obtained from the following sources.
    • DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link

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