Overview | |
| Organism | Human (Homo sapiens), 4 years old, Male, White REF |
| Source/Tissue | Brain, Cerebellum, Desmoplastic Cerebellar Medulloblastoma (Jacobsen et al., 1985) |
| Morphology | Polygonal REF |
| Cell type | |
| Growth mode | Adherent, monolayer REF |
| Doubling time | ≈ 34 hours REF |
| Life span | Infinite, continuous culture (Jacobsen et al., 1985) |
| Karyotype | Hypertetraploid (modal number between 93 and 99) REF |
| Transformed | Naturally transformed (anchorage independent growth) |
| Tumorigenic | |
| Drug resistance | |
| Biosafety Level | 1 REF (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | |
| Applications | Suitable transfection host REF Medulloblastoma research |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line DAOY was established from Desmoplastic Cerebellar Medulloblastoma tumor taken from a 4 year old boy. (Jacobsen et al., 1985)
Recommended propagation method
Culture medium:
Eagle’s Minimum Essential Medium (+ 2mM Glutamine) + 10% FBS REF
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C REF
Split ratio: 1:4 to 1:6 REF
Split frequency:
Harvesting time: Subconfluent culture (≈ 80% ) REF
Split Method: Trypsin/EDTA REF
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ?
Availability
- American Type Culture Collection (ATCC) : Product Link
REFERENCES
- Jacobsen et al., 1985. Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice. J Neuropathol Exp Neurol. 44(5), 472-485. PMID-2993532; Full Text Link: academic.oup.
- American Type Culture Collection (ATCC)
