Cell Line : DAOY


OrganismHuman (Homo sapiens), 4 years old, Male, White REF
Source/TissueBrain, Cerebellum, Desmoplastic Cerebellar Medulloblastoma (Jacobsen et al., 1985)
MorphologyPolygonal REF
Cell type 
Growth modeAdherent, monolayer REF
Doubling time≈ 34 hours REF
Life spanInfinite, continuous culture (Jacobsen et al., 1985)
KaryotypeHypertetraploid (modal number between 93 and 99) REF
TransformedNaturally transformed (anchorage independent growth)
Drug resistance 
Biosafety Level1 REF (May differ in your country, follow the guidelines of your institute/country)
Gene Expression 
ApplicationsSuitable transfection host  REF
Medulloblastoma research
Related cell lines 

Cell line isolation information

The cell line DAOY was established from Desmoplastic Cerebellar Medulloblastoma tumor taken from a 4 year old boy. (Jacobsen et al., 1985)

Recommended propagation method

Culture medium:
Eagle’s Minimum Essential Medium (+ 2mM Glutamine) + 10% FBS REF

Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C REF
Split ratio: 1:4 to 1:6 REF
Split frequency:
Harvesting time: Subconfluent culture (≈ 80% ) REF
Split Method: Trypsin/EDTA REF

Recommended Cryopreservation Method

Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ?



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