Cell Line : D341 Med


OrganismHuman (Homo sapiens), 3.5 years old, Male (Friedman et al., 1988)
Source/TissueBrain, Cerebellum, Medulloblastoma (Friedman et al., 1988)
MorphologyIrregular shaped floating aggregates REF
Cell type 
Growth modeSuspension/Semi-adherent REF
Doubling time 
Life spanInfinite, continuous culture (Friedman et al., 1988)
KaryotypeHyperdiploid (modal number = 49; range = 44 to 50) REF
TransformedNaturally transformed (anchorage independent growth)
TumorigenicTumorigenic in nude mice REF
Drug resistance 
Biosafety Level1 REF (May differ in your country, follow the guidelines of your institute/country)
Gene Expression 

3D cell culture REF
Neuroscience REF

Related cell lines 

Cell line isolation information

The cell line D341 Med was established from a Medulloblastoma tumor taken from a 3.5 year old male. (Friedman et al., 1988)

Recommended propagation method

Culture medium:
Eagle’s Minimum Essential Medium (+ 2mM Glutamine) + 10% FBS REF

Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C REF
Split ratio:
Split frequency:
Harvesting time: Subconfluent culture (≈ 80% )
Split Method: Dilution REF

Recommended Cryopreservation Method

Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ?



  • Friedman et al., 1988. Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am J Pathol. 130(3), 472-484. PMID-3279793; Full Text Link: PMC
  • American Type Culture Collection (ATCC)

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