Overview | |
| Organism | Human (Homo sapiens), 3.5 years old, Male (Friedman et al., 1988) |
| Source/Tissue | Brain, Cerebellum, Medulloblastoma (Friedman et al., 1988) |
| Morphology | Irregular shaped floating aggregates REF |
| Cell type | |
| Growth mode | Suspension/Semi-adherent REF |
| Doubling time | |
| Life span | Infinite, continuous culture (Friedman et al., 1988) |
| Karyotype | Hyperdiploid (modal number = 49; range = 44 to 50) REF |
| Transformed | Naturally transformed (anchorage independent growth) |
| Tumorigenic | Tumorigenic in nude mice REF |
| Drug resistance | |
| Biosafety Level | 1 REF (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | |
| Applications | |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line D341 Med was established from a Medulloblastoma tumor taken from a 3.5 year old male. (Friedman et al., 1988)
Recommended propagation method
Culture medium:
Eagle’s Minimum Essential Medium (+ 2mM Glutamine) + 10% FBS REF
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C REF
Split ratio:
Split frequency:
Harvesting time: Subconfluent culture (≈ 80% )
Split Method: Dilution REF
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ?
Availability
- American Type Culture Collection (ATCC) : Product Link
REFERENCES
- Friedman et al., 1988. Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am J Pathol. 130(3), 472-484. PMID-3279793; Full Text Link: PMC
- American Type Culture Collection (ATCC)
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