| CELL LINE ORIGIN | |
| Organism | Chinese Hamster, Female |
| Source/Tissue | Ovary |
| Disease | Healthy |
| Year of origin | 1957 |
| CELL LINE FEATURES | |
| Morphology | Epithelial-like/Fibroblast-like |
| Cell type | Epithelial |
| Growth mode | Adherent, Monolayer |
| Doubling time | ? |
| Life span | Infinite (continuous culture) |
| Transformed | ? |
| Tumorigenic | ? |
| Drug resistance | ? |
| Products | ? |
| Karyotype | Hypodiploid, modal chromosome number: 20 |
| Proline synthesis | Deficient in proline synthesis |
| GENE EXPRESSION | |
| APPLICATIONS | |
| Production of recombinant therapeutic proteins | |
| CULTURE MAINTENANCE AND STORAGE | |
| Culture conditions and handling | |
| Biosafety Level | 2 REF (It may differ in your country, follow the guidelines of your institute/country) |
| Plating efficiency | ? |
| Temperature | 37°C |
| Atmosphere | Humidified incubator, 95% air + 5% CO2 |
| Base medium | Ham’s F12 (2mM Glutamine) |
| Complete culture medium | Ham’s F12 (2mM Glutamine) + 10% FBS |
| Medium Renewal | 2 to 3 times per week |
| Subculturing procedure | |
| Harvesting time | Subconfluent culture |
| Split method | Trypsin or Trypsin/EDTA |
| Split ratio | 1:3 to 1:8 |
| Seeding density | 1 – 3 x 104 cells/cm² |
| Split frequency | |
| Cryopreservation | |
| Cryomedium | Complete culture medium + 5% DMSO OR Growth medium + 20% FBS + 10% DMSO |
| Storage conditions | Liquid nitrogen vapor phase |
| AVAILABILITY / SOURCE | |
American Type Culture Collection (ATCC) | |
Public Health England Culture Collections | Product page link |
Sigma-Aldrich | |
Japanese Collection of Research Bioresources (JCRB) | |
Cell Bank Australia | |
ACCEGEN BIOTECHNOLOGY | |
Abcam | |
ThermoFisher Scientific | |
| CELL LINE IDENTIFICATIONS | |
| Synonyms | |
| Remarks | |
| Related cell lines | |
References
- Puck et al., 1958. Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med. 108(6), 945-956. PMID-13598821; Full-Text Links: rupress, PMC2136918
