Cell Line: CHO

CELL LINE ORIGIN
OrganismChinese Hamster, Female
Source/TissueOvary
DiseaseHealthy
Year of origin 1957
CELL LINE FEATURES
MorphologyEpithelial-like/Fibroblast-like
Cell typeEpithelial
Growth modeAdherent, Monolayer
Doubling time?
Life spanInfinite (continuous culture)
Transformed?
Tumorigenic?
Drug resistance?
Products?
KaryotypeHypodiploid, modal chromosome number: 20
Proline synthesisDeficient in proline synthesis
GENE EXPRESSION
  
APPLICATIONS
 Production of recombinant therapeutic proteins
CULTURE MAINTENANCE AND STORAGE
Culture conditions and handling
Biosafety Level2 REF (It may differ in your country, follow the guidelines of your institute/country)
Plating efficiency?
Temperature37°C
AtmosphereHumidified incubator, 95% air + 5% CO2
Base mediumHam’s F12 (2mM Glutamine)
Complete culture mediumHam’s F12 (2mM Glutamine) + 10% FBS
Medium Renewal2 to 3 times per week
Subculturing procedure
Harvesting timeSubconfluent culture
Split methodTrypsin or Trypsin/EDTA
Split ratio1:3 to 1:8
Seeding density1 – 3 x 104 cells/cm²
Split frequency 
Cryopreservation
CryomediumComplete culture medium + 5% DMSO
OR
Growth medium + 20% FBS + 10% DMSO
Storage conditions
Liquid nitrogen vapor phase
AVAILABILITY / SOURCE

American Type Culture Collection (ATCC)

 

Public Health England Culture Collections

Product page link

Sigma-Aldrich

 

Japanese Collection of Research Bioresources (JCRB)

 

Cell Bank Australia

 

ACCEGEN BIOTECHNOLOGY

 

Abcam

 

ThermoFisher Scientific

 
 CELL LINE IDENTIFICATIONS
Synonyms
 
Remarks 
Related cell lines 

 

References

  • Puck et al., 1958. Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med. 108(6), 945-956. PMID-13598821; Full-Text Links: rupress, PMC2136918

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