Category: Lab Notes: Bioinformatics

BLAST and GenBank: How to Compare DNA Sequences

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BLAST and GenBank work together to make DNA sequence analysis easier. Learn how BLAST compares nucleotide sequences, how to evaluate BLAST hits, and how GenBank records help identify and interpret DNA sequences.

How to Access GenBank Programmatically: APIs, E-utilities, and Bioinformatics Tools

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Learn how to access GenBank programmatically using NCBI E-utilities, Entrez Direct, Biopython, NCBI Datasets, APIs, and other bioinformatics tools.

GenBank Flat File Format Explained with Examples

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Understand the GenBank flat file format with practical examples of LOCUS, ACCESSION, VERSION, FEATURES, qualifiers, ORIGIN, and nucleotide sequence data.

GenBank File Format: A Complete Guide

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Learn how the GenBank file format organizes nucleotide sequences, accession information, annotations, biological features, qualifiers, references, and sequence data.

GenBank Sequence Submission Tools: Preparing and Validating Your Data

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Learn how to prepare and validate nucleotide sequence data for GenBank submission, including FASTA files, source metadata, annotation, Submission Portal workflows, validation, and common submission problems.

How to Submit DNA Sequences to GenBank

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Learn how to submit DNA sequences to GenBank, including sequence preparation, biological metadata, annotation, validation, submission tools, accession numbers, and data-release considerations.

NCBI Entrez Nucleotide: Searching and Retrieving GenBank Sequences

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Learn how NCBI Entrez Nucleotide is used to search and retrieve GenBank sequence records, including searches by accession number, organism, gene, and sequence type.

What Is GenBank? History, Structure, and Importance

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GenBank is a major public nucleotide sequence database maintained by NCBI. Learn about its history, structure, sequence records, accession numbers, annotations, uses, and importance in modern bioinformatics and molecular biology.

Understanding a GenBank Record: A Complete Guide

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Learn how to understand a GenBank record, including its accession number, definition, organism information, references, FEATURES section, annotations, and nucleotide sequence.

GenBank

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GenBank is a major public nucleotide sequence database used in genomics, molecular biology, bioinformatics, evolutionary research, and biotechnology. Explore its records, accession numbers, annotations, sequence data, submission process, and major applications.

GenBank Accession Numbers: Types, Structure, and Uses

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Learn what GenBank accession numbers are, how accession.version identifiers work, the different types of GenBank accessions, and how researchers use them to find, retrieve, cite, and analyze nucleotide sequences.

GenBank Sequence Annotation: Genes, CDS, RNA, and Other Features

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Understand GenBank sequence annotation, including genes, CDS, mRNA, tRNA, rRNA, feature locations, qualifiers, and how annotations connect biological information to nucleotide sequences.

GenBank Database Structure: How GenBank Organizes Sequence Data

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Learn how GenBank organizes nucleotide sequence data into structured records containing accession numbers, annotations, taxonomy, references, sequence features, and nucleotide sequences.

Types of Sequence Data in GenBank: Genomic, mRNA, WGS, TSA, and More

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Learn about the major types of sequence data in GenBank, including genomic DNA, mRNA, tRNA, rRNA, organelle genomes, WGS, TSA, TLS, and environmental sequences.

Whole Genome Shotgun (WGS) Data in GenBank

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Learn how Whole Genome Shotgun (WGS) data are represented in GenBank, including WGS sequences, accession identifiers, annotations, genome assemblies, data retrieval, and applications in genomic research.