Category: Lab Notes: Biotechniques
Confocal Microscope
A confocal microscope represents a sophisticated advancement in optical imaging technology, providing researchers with the ability to capture high-resolution, three-dimensional…
Electron Microscope
An electron microscope is a sophisticated scientific instrument that uses beams of electrons instead of light to create images of…
Inverted Microscope
An inverted microscope is a specialized form of light microscope where the light source and condenser are positioned above the…
Visium Spatial Gene Expression
Visium Spatial Gene Expression, developed by 10x Genomics, is a powerful spatial transcriptomics platform that enables researchers to analyze gene…
CellScape
CellScape is an innovative spatial biology platform developed by Parse Biosciences that combines single-cell RNA sequencing with spatial context preservation….
PhenoImager HT (Vectra Polaris)
PhenoImager HT (formerly Vectra Polaris) represents a significant advancement in spatial biology analysis, offering high-throughput multiplex imaging capabilities for comprehensive…
MERFISH (Multiplexed Error-Robust Fluorescence In Situ Hybridization)
MERFISH (Multiplexed Error-Robust Fluorescence In Situ Hybridization) is a powerful spatial transcriptomics technology that enables highly multiplexed imaging of RNA…
Xenium In Situ
Xenium In Situ is 10x Genomics’ advanced spatial transcriptomics platform that enables high-resolution, single-cell analysis of gene expression directly in…
Antibody Phage Display
Antibody phage display is a powerful in vitro technique used to generate and select antibodies with high affinity and specificity…
Protocol: Analysis of Protein Homodimerization via Differential Tagging and Co-Immunoprecipitation in Mammalian Cells
Overview Homodimerization implies that identical or nearly identical protein monomers associate under physiological conditions. By engineering two expression constructs encoding…
Förster Resonance Energy Transfer (FRET)
Förster Resonance Energy Transfer (FRET) is a highly sensitive and non-invasive technique used to study molecular interactions, particularly protein–protein interactions,…
Bimolecular Fluorescence Complementation (BiFC)
Bimolecular Fluorescence Complementation (BiFC) is a powerful technique used to study protein–protein interactions in living cells by exploiting the reconstitution…
Proximity Labeling
Proximity labeling is a powerful and versatile biochemical approach used to identify and study protein–protein interactions and the molecular composition…
Integrative Omics
Integrative omics is an emerging and transformative approach in modern biology that combines multiple layers of molecular data to achieve…
Metabolomics
Metabolomics is a cutting-edge field within systems biology and molecular biology, offering real-time insights into biochemical processes by analyzing metabolite…