Category: Lab Notes: Biotechniques
Maxam–Gilbert Sequencing
Maxam–Gilbert sequencing, also known as chemical sequencing, is one of the first DNA sequencing methods ever developed. Introduced in 1977…
First-Generation Sequencing
First-Generation Sequencing encompasses the earliest set of DNA sequencing technologies developed in the 1970s and 1980s, which provided the first…
Third-Generation Sequencing (TGS)
Third-Generation Sequencing (TGS) refers to a set of advanced DNA sequencing technologies that enable the direct reading of long DNA…
DNA-Based Tools
DNA-based tools have transformed the landscape of biotechnology, offering unprecedented precision, scalability, and versatility across fields such as medicine, agriculture,…
DNA Digital Data Storage
DNA digital data storage is an emerging technology that uses the molecular structure of DNA to encode, store, and retrieve…
DETECTR (DNA Endonuclease Targeted CRISPR Trans Reporter)
DETECTR (DNA Endonuclease Targeted CRISPR Trans Reporter) is a CRISPR-based molecular diagnostic platform designed for the sensitive and specific detection…
SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing)
SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) is a nucleic acid detection platform that leverages the collateral cleavage activity of CRISPR-associated…
Sanger Sequencing
Sanger sequencing, also known as the chain-termination method, is a DNA Sequencing technique developed by Frederick Sanger and his colleagues in 1977….
Golden Gate Assembly
Golden Gate Assembly is a powerful and efficient molecular cloning method that allows for the precise and simultaneous assembly of…
DNA Sequencing
DNA sequencing is the process of determining the exact order of nucleotides—adenine (A), thymine (T), cytosine (C), and guanine (G)—within…
Immortalized Cell Lines as Alternatives to Primary Cultures
Immortalized cell lines offer a practical and widely used alternative to primary cell cultures, especially when consistency, scalability, and ease…
Challenges in Primary Tissue Processing for Cell Isolation
The preparation of primary human and animal tissues for cell isolation presents numerous challenges that can impact the quality, yield,…
SenSCOUT (Senescence Subtype Classifier Based on Observable Unique Phenotypes)
SenSCOUT (Senescence Subtype Classifier Based on Observable Unique Traits) is an advanced tool developed to identify and classify distinct subtypes…
Positron Emission Tomography (PET)
Positron Emission Tomography (PET) is a sophisticated nuclear imaging technique that provides functional information about biological processes in the body,…
Computed Tomography (CT) Scan
Computed Tomography (CT) scan, also known as CAT scan (Computed Axial Tomography), is a widely used medical imaging technique that…