- Synonyms:
- 293 c18
- 293c18
- HEK 293 c18
- HEK-293 c18
- HEK293EBNA
- HEK 293 EBNA
- HEK293E
- HEK/EBNA
- HEK-EBNA
- HEK293-EBNA1
- 293/EBNA-1
- 293EBNA
- EBNA-293
- EBNA293
- Description:
- Keywords: EBNA1 expressing HEK-293 cells
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial
- Growth Mode: Adherent
- Morphology: Epithelial-like
- Applications:
- 3D cell culture
- Suitable for transient transfection (High transfection efficiency)
- Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- DMEM (4.5 g/L glucose, 4 mM L-glutamine, 1.5 g/L sodium bicarbonate) + 10% FBS + G418 (0.25 mg/ml)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO₂, 95% Air
- Subculturing:
- Cell dissociation procedure: 0.25% trypsin – 0.03% EDTA
- Harvesting time: 80% confluence culture
- Subcultivation Ratio: 1:4 to 1:10
- Medium Renewal: Twice per week
- Seeding densities:
- Cell dissociation procedure: 0.25% trypsin – 0.03% EDTA
- Cryopreservation
- Growth medium + 5% DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion (CLS Cell Lines Service): Product link
- References:
- https://www.atcc.org/products/crl-10852
- https://www.cytion.com/HEK293-EBNA/300264
- Murphy et al. 1992. cDNA expression cloning in human cells using the pλDR2 episomal vector system. Methods 4(2):111–131. Full-Text Links: Sciencedirect
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Fetal Bovine Serum)
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