Cell Line: HEK 293 STF

Description: HEK 293 cells express luciferase reporter under the control of 7 LEF/TCF binding sites

• Synonyms: HEK 293 STF, 293 STF
  
• Keywords: HEK 293 cells express luciferase reporter under the control of 7 LEF/TCF binding sites
  
• Culture Type: Cell line
• Origin/Source:
  • Organism:
    • Species: Human (Homo sapiens)
    • Age: Fetus
  • Tissue of Origin: Embryonic Kidney
• Cell line features:
  • Morphology: Epithelial-like
    
  • Cell Type: Epithelial
    
  • Growth Mode: Adherent
• Applications:
  • Luciferase reporter assay
  • High-throughput screening
    
• Handling and maintenance
  • Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
  • GMO Status: GMO1
  • Complete culture medium: 
    • DMEM + 20% FBS + G418 (200 µg/ml)
  • Culture conditions: 
    • Temperature: Humidified 37°C incubator
    • Atmosphere: 5% CO₂, 95% Air
  • Subculturing:
    • Cell dissociation procedure: 0.05% Trypsin/EDTA or Trypsin
    • Harvesting time: 80% confluence culture
    • Subcultivation Ratio: 1:3 to 1:5
    • Medium Renewal: 2 to 3 times per week
      
    • Seeding densities: 3.0 x 10⁴ to 5.0 x10⁴ cells/cm²
      
  • Cryopreservation
    • Growth medium + 50% FBS + 10% DMSO
    • Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer

• Availability:
  • American Type Culture Collection (ATCC): Product link
• References: 
  • https://www.atcc.org/products/crl-3249
  • Xu Q et al. 2004. Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair. Cell. 116(6), 883-95. PMID-15035989; Full-Text Links: Cell (Download PDF)
• Abbreviations
  • DMEM (Dulbecco’s Modified Eagle’s Medium)
  • FBS (Foetal Bovine Serum)
  • GMO1 (Genetically Modified Organism Class 1)