Cell Line: A-431

CELL LINE ORIGIN
OrganismHuman (Homo sapiens), 85 years old female
Source/TissueSkin, Epidermis
DiseaseEpidermoid Carcinoma
Year of origin?
CELL LINE FEATURES
MorphologyEpithelial-like
Cell type?
Growth modeAdherent, Monolayer
Doubling time?
Life spanInfinite (continuous culture)
Transformed?
TumorigenicYes, in immunosuppressed mice
Drug resistance?
Products?
KaryotypeHypertriploid
  
GENE EXPRESSION
  
APPLICATIONS
 3D cell culture
Cancer research
High-throughput screening
Toxicology
Immuno-oncology
CULTURE MAINTENANCE AND STORAGE
Culture conditions and handling
Biosafety Level1 REF (It may differ in your country, follow the guidelines of your institute/country)
Plating efficiency?
Temperature37°C
AtmosphereHumidified incubator, 95% air + 5% CO2
Base mediumDMEM (High Glucose)
Complete culture mediumDMEM + 10% FBS
Medium RenewalEvery 2 to 3 days
Subculturing procedure
Harvesting timeSubconfluent culture
Split methodTrypsin or Trypsin/EDTA
Split ratio1:3 to 1:8
Seeding density 
Split frequency 
Cryopreservation
Cryomedium

Complete culture medium + 5% DMSO
OR

Culture medium + 20% FBS * + 10% DMSO (* One can increase FBS up to 50%)

Storage conditions
Liquid nitrogen vapor phase
AVAILABILITY / SOURCE

American Type Culture Collection (ATCC)

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Public Health England Culture Collections

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Sigma-Aldrich

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Japanese Collection of Research Bioresources (JCRB)

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Cell Bank Australia

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ACCEGEN BIOTECHNOLOGY

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Abcam

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Remarks 
Related cell lines 

 

References

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