| CELL LINE ORIGIN | |
| Organism | Human (Homo sapiens), 53 years old, White Male |
| Source/Tissue | Lung, Carcinoma |
| Year of origin | ? |
| CELL LINE FEATURES | |
| Morphology | Epithelial-like |
| Cell type | ? |
| Growth mode | Adherent, Monolayer |
| Doubling time | ? |
| Life span | Infinite (continuous culture) |
| Transformed | ? |
| Tumorigenic | Yes, in nude mice |
| Drug resistance | ? |
| Products | ? |
| Karyotype | Hypotriploid to hypertriploid |
| GENE EXPRESSION | |
| APPLICATIONS | |
| CULTURE MAINTENANCE AND STORAGE | |
| Culture conditions and handling | |
| Biosafety Level | 1 REF (It may differ in your country, follow the guidelines of your institute/country) |
| Plating efficiency | ? |
| Temperature | 37°C |
| Atmosphere | Humidified incubator, 95% air + 5% CO2 |
| Base medium | RPMI 1640 |
| Complete culture medium | RPMI 1640 + 10% FBS |
| Medium Renewal | Twice per week |
| Subculturing procedure | |
| Harvesting time | Subconfluent culture |
| Split method | Trypsin or Trypsin/EDTA |
| Split ratio | 1:2 to 1:6 |
| Seeding density | 2 x 106 cells/T-75 flask |
| Split frequency | Every fourth or fifth day |
| Cryopreservation | |
| Cryomedium | Complete culture medium + 5% DMSO |
| Storage conditions | Liquid nitrogen vapor phase |
| AVAILABILITY / SOURCE | |
American Type Culture Collection (ATCC) | Product page link |
Public Health England Culture Collections | |
Sigma-Aldrich | |
Japanese Collection of Research Bioresources (JCRB) | |
| ACCEGEN BIOTECHNOLOGY | Product page link |
| DSMZ | Product page link |
| Remarks | |
| Related cell lines | |
References
- Giard et al., 1973. In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors. J Natl Cancer Inst. 51 (5), 1417-23. PMID-4357758 | Full Text – Oxford University Press
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