| Overview | |
| Organism | Canine, Dog (Canis familiaris), 8 years, Female, Strain Golden retriever |
| Source/Tissue | Tumor, Unknown tissue |
| Morphology | Fibroblast-like |
| Cell type | Fibroblast |
| Growth mode | Adherent |
| Doubling time | ? |
| Life span | ? |
| Karyotype | ? |
| Transformed | ? |
| Tumorigenic | ? |
| Drug resistance | ? |
| Biosafety Level | ? |
| Gene Expression | ? |
| Products | ? |
| Applications | Efficacy testing |
| Remarks | ? |
| Related cell lines |
Cell line isolation information
The cell line A-72 was established from the tumor taken from the left thigh of 8-year-old female Golden Retriever which had a previous history of mammary adenocarcinoma.
Recommended propagation method
Culture medium: Leibovitz’s L-15 Medium (+2mM Glutamine) + 10% FBS
Culture conditions: Incubator with no CO2 at 37°C. Free gas exchange with atmospheric air necessary.
Split ratio: 1:2 to 1:4 (seeding at 2-5 x 103 cells/cm²)
Split frequency: ?
Harvesting time: Subconfluent culture
Split Method: Trypsin/EDTA
Recommended Cryopreservation Method
Freeze medium:
Storage temperature: Liquid nitrogen vapor phase
Cell density:
Availability (Where to purchase the Cell Line)
- American Type Culture Collection (ATCC): Catalog No. : CRL-6295TM
- Sigma-Aldrich: Catalog No.: 89050908
- Cell Bank Australia: Code. : 89050908
- Public Health England Culture Collections, Catalogue No.: 89050908
- Cell Line Service (CLS): Artikelnummer: 602398
- Bioresource Collection and Research Center (BCRC): BCRC Number: 60480
References
- American Type Culture Collection (ATCC)
- Public Health England Culture Collections
- Expasy
- Binn et al., 1980. Establishment of a canine cell line: derivation, characterization, and viral spectrum. Am J Vet Res. 41(6), 855-860. PMID-6254399
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