Cell Line : A-72

Overview 
OrganismCanine, Dog (Canis familiaris), 8 years, Female, Strain Golden retriever
Source/TissueTumor, Unknown tissue
MorphologyFibroblast-like
Cell typeFibroblast
Growth modeAdherent
Doubling time?
Life span?
Karyotype?
Transformed?
Tumorigenic?
Drug resistance?
Biosafety Level?
Gene Expression?
Products?
ApplicationsEfficacy testing
Remarks?
Related cell lines 

Cell line isolation information

The cell line A-72 was established from the tumor taken from the left thigh of 8-year-old female Golden Retriever which had a previous history of mammary adenocarcinoma.

Recommended propagation method

Culture medium: Leibovitz’s L-15 Medium (+2mM Glutamine) + 10% FBS
Culture conditions: Humidified incubator with no CO2 at 37°C. Free gas exchange with atmospheric air necessary.
Split ratio: 1:2 to 1:4 (seeding at 2-5 x 103 cells/cm²)
Split frequency: ?
Harvesting time: Subconfluent culture
Split Method: Trypsin/EDTA

Recommended Cryopreservation Method

Freeze medium:
Storage temperature: Liquid nitrogen vapor phase
Cell density: 

Availability (Where to purchase the Cell Line)

References

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