| CELL LINE ORIGIN | |
| Organism | Rat (Rattus norvegicus) |
| Strain | DB1X |
| Age | 14–17 day old embryo |
| Source/Tissue | Medial and intimal layers of thoracic aorta of Embryo (Kimes and Brandt, 1976) |
| Disease | Normal |
| Year of origin | 1976 |
| CELL LINE FEATURES | |
| Morphology | Myoblast-like |
| Cell type | Exhibits many properties characteristic of smooth muscle cells |
| Growth mode | Adherent, Monolayer |
| Doubling time | ≈29 hours |
| Life span | Infinite (continuous culture) |
| Transformed | ? |
| Tumorigenic | ? |
| Drug resistance | ? |
| Products | Myokinase Creatine phosphokinase Myosin (ATCC) |
| Karyotype | Rat karyotype |
| GENE EXPRESSION | |
| PDGF α receptor | No expression (Rao, et al., 1997) |
| PDGF B chain mRNA | No expression (Rao, et al., 1997) |
| Desmin | No expression (Rao, et al., 1997) |
| tropoelastin mRNA | Low expression (Rao, et al., 1997) |
| Smooth muscle α-actin | Yes (Rao, et al., 1997) |
| Vimentin | Yes (Rao, et al., 1997) |
| Non-Muscle Myosin | Yes (Rao, et al., 1997) |
| APPLICATIONS | |
| Suitable transfection host A suitable model system to study vascular smooth muscle cells (VSMCs) | |
| CULTURE MAINTENANCE AND STORAGE | |
| Culture conditions and handling | |
| Biosafety Level | 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country) |
| Plating efficiency | ? |
| Temperature | 37°C |
| Atmosphere | Humidified incubator, 95% air + 5% CO2 |
| Base medium | DMEM |
| Complete culture medium | DMEM + 10% FBS |
| Medium Renewal | Every 3 to 4 days |
| Subculturing procedure | |
| Harvesting time | Subconfluent culture |
| Split method | Trypsin/EDTA |
| Split ratio | 1:3 to 1:6 |
| Seeding density | |
| Split frequency | Once or twice a week |
| Cryopreservation | |
| Cryomedium | Culture medium + 20% FBS + 10% DMSO |
| Storage conditions | Liquid nitrogen vapor phase |
| AVAILABILITY / SOURCE | |
American Type Culture Collection (ATCC) | Product page link |
DSMZ | Product page link |
| CELL LINE IDENTIFICATIONS | |
| Synonyms | A10, A-10 |
| Remarks | |
| Related cell lines | A7r5 |
References
- Kimes & Brandt, 1976. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp Cell Res. 98 (2), 349-366. PMID-943301 | Full Text – Sciencedirect
- https://www.atcc.org/products/crl-1476
- https://cellbank.nibiohn.go.jp/~cellbank/en/search_res_det.cgi?RNO=IFO50519
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