Cell Line : A-10

CELL LINE ORIGIN
OrganismRat (Rattus norvegicus)
Strain
DB1X
Age
14–17 day old embryo
Source/TissueMedial and intimal layers of thoracic aorta of Embryo (Kimes and Brandt, 1976)
DiseaseNormal
Year of origin1976
CELL LINE FEATURES
MorphologyMyoblast-like
Cell typeExhibits many properties characteristic of smooth muscle cells
Growth modeAdherent, Monolayer
Doubling time≈29 hours
Life spanInfinite (continuous culture)
Transformed?
Tumorigenic?
Drug resistance?
ProductsMyokinase
Creatine phosphokinase
Myosin (ATCC)
KaryotypeRat karyotype
  
GENE EXPRESSION
PDGF α receptorNo expression (Rao, et al., 1997)
PDGF B chain mRNA No expression (Rao, et al., 1997)
DesminNo expression (Rao, et al., 1997)
tropoelastin mRNALow expression (Rao, et al., 1997)
Smooth muscle α-actinYes (Rao, et al., 1997)
VimentinYes (Rao, et al., 1997)
Non-Muscle MyosinYes (Rao, et al., 1997)
APPLICATIONS
 Suitable transfection host
A suitable model system to study vascular smooth muscle cells (VSMCs)
CULTURE MAINTENANCE AND STORAGE
Culture conditions and handling
Biosafety Level1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
Plating efficiency?
Temperature37°C
AtmosphereHumidified incubator, 95% air + 5% CO2
Base mediumDMEM
Complete culture mediumDMEM + 10% FBS
Medium RenewalEvery 3 to 4 days
Subculturing procedure
Harvesting timeSubconfluent culture
Split methodTrypsin/EDTA
Split ratio1:3 to 1:6
Seeding density 
Split frequency Once or twice a week
Cryopreservation
Cryomedium

Culture medium + 20% FBS + 10% DMSO
OR
Complete Culture medium + 5% DMSO

Storage conditions
Liquid nitrogen vapor phase
AVAILABILITY / SOURCE

American Type Culture Collection (ATCC)

Product page link

DSMZ

Product page link
CELL LINE IDENTIFICATIONS
Synonyms
 A10, A-10
Remarks 
Related cell linesA7r5

 

References

  • Kimes & Brandt, 1976. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp Cell Res. 98 (2), 349-366. PMID-943301 | Full Text – Sciencedirect
  • https://www.atcc.org/products/crl-1476
  • https://cellbank.nibiohn.go.jp/~cellbank/en/search_res_det.cgi?RNO=IFO50519

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