| Overview | |
| Organism | Rat (Rattus norvegicus), Strain DB1X |
| Source/Tissue | Embryo, Thoracic aorta, Medial and intimal layers |
| Morphology | Myoblast-like |
| Cell type | Exhibit many properties characteristic of smooth muscle cells |
| Growth mode | Adherent growing in monolayers |
| Doubling time | Approximately 29 hours |
| Life span | ? |
| Karyotype | Rat karyotype |
| Transformed | ? |
| Tumorigenic | ? |
| Drug resistance | ? |
| Biosafety Level | 1 REF |
| Gene Expression | ? |
| Products | ? |
| Applications | ? |
| Remarks | ? |
| Related cell lines |
Cell line isolation information
The cell line A10 was established from the thoracic aorta of DBIX embryonic rat in 1976.
Recommended propagation method
Culture medium: DMEM + 10% FCS
Culture conditions: Humidified incubator with 5% CO2 at 37°C
Split ratio: 1:3 to 1:6
Split frequency: Once or twice a week
Harvesting time: Subconfluent culture (90% confluent)
Split Method: Trypsin/EDTA
Recommended Cryopreservation Method
Freeze medium: 70% culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Cell density: 0.5-1 x 106 cells/ml, 1ml/ampoule
Availability
> American Type Culture Collection (ATCC) : Catalog No. : CRL-1620TM
> Public Health England Culture Collections: Catalogue No.: 88062428
>Common Access to Biological Resources and Information (CABRI): Catalog No. : ICLC HTL97013
> Japanese Collection of Research Bioresources (JCRB): Catalog No.: JCRB0228
> Sigma-Aldrich: Catalog No.: 88062428
References
> American Type Culture Collection (ATCC)
> Common Access to Biological Resources and Information (CABRI)
> Japanese Collection of Research Bioresources (JCRB)
> Kimes & Brandt 1976. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp Cell Res. 98(2), 349-366. PMID-943301 | Full Text – Sciencedirect
